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The physiological oxygen concentration of many tissues is far lower than that in which cells are typically cultured in vitro and this may inadvertently influence the proliferation and differentiation potential of many cell types. Muscle derived stem cells, known as satellite cells are responsible for the maintenance and repair of muscle tissue post natally and in vivo would be exposed to oxygen concentrations of 2 “5%. Relatively few studies describe the function of these cells in large animal models and here we investigate the influence oxygen concentration has on modulating porcine muscle derived stem cell fate.
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